Genetically engineered bacterium for producing 3-hydracrylic acid and application thereof
The invention belongs to the technical field of metabolic engineering and synthetic biology, and relates to a genetically engineered bacterium for producing 3-hydracrylic acid and application of the genetically engineered bacterium. According to the saccharomyces cerevisiae recombinant strain, an ex...
Gespeichert in:
Hauptverfasser: | , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention belongs to the technical field of metabolic engineering and synthetic biology, and relates to a genetically engineered bacterium for producing 3-hydracrylic acid and application of the genetically engineered bacterium. According to the saccharomyces cerevisiae recombinant strain, an exogenous modified malonyl-coenzyme A reductase gene MCR is positioned in a saccharomyces cerevisiae mitochondria, two copies are expressed on a chromosome, and an endogenous mitochondrial NADP specific isocitrate dehydrogenase coding gene IDP1 and a mitochondrial NADH kinase coding gene POS5 are overexpressed. The recombinant saccharomyces cerevisiae strain with high yield of 3-HP is constructed through the transformation, the yield of the recombinant saccharomyces cerevisiae strain reaches 5.2 g/L in shake flask fermentation, and the yield of the recombinant saccharomyces cerevisiae strain reaches 36.3 g/L when the recombinant saccharomyces cerevisiae strain is subjected to fed-batch fermentation in a 5L fermentati |
---|