Genetically engineered bacterium for producing 3-hydracrylic acid and application thereof

The invention belongs to the technical field of metabolic engineering and synthetic biology, and relates to a genetically engineered bacterium for producing 3-hydracrylic acid and application of the genetically engineered bacterium. According to the saccharomyces cerevisiae recombinant strain, an ex...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG YIMING, LIU ZIHE, SU MO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of metabolic engineering and synthetic biology, and relates to a genetically engineered bacterium for producing 3-hydracrylic acid and application of the genetically engineered bacterium. According to the saccharomyces cerevisiae recombinant strain, an exogenous modified malonyl-coenzyme A reductase gene MCR is positioned in a saccharomyces cerevisiae mitochondria, two copies are expressed on a chromosome, and an endogenous mitochondrial NADP specific isocitrate dehydrogenase coding gene IDP1 and a mitochondrial NADH kinase coding gene POS5 are overexpressed. The recombinant saccharomyces cerevisiae strain with high yield of 3-HP is constructed through the transformation, the yield of the recombinant saccharomyces cerevisiae strain reaches 5.2 g/L in shake flask fermentation, and the yield of the recombinant saccharomyces cerevisiae strain reaches 36.3 g/L when the recombinant saccharomyces cerevisiae strain is subjected to fed-batch fermentation in a 5L fermentati