Preparation method and application of RBC antibody magnetic beads

The invention discloses a preparation method and application of an RBC antibody magnetic bead, carboxyl magnetic particles and an RBC antibody are coupled to prepare the RBC antibody magnetic bead, and the preparation method specifically comprises the following steps: step 1, a carboxyl magnetic bea...

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Bibliographische Detailangaben
Hauptverfasser: HAN SHUANG, MA AIYING, XIA ZHENWEI, LIU ZHENSHI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a preparation method and application of an RBC antibody magnetic bead, carboxyl magnetic particles and an RBC antibody are coupled to prepare the RBC antibody magnetic bead, and the preparation method specifically comprises the following steps: step 1, a carboxyl magnetic bead concentrated solution is fully and uniformly mixed; after fully and uniformly mixing, taking out a carboxyl magnetic bead concentrated solution and putting into a reaction bottle; 2, placing the reaction container in a magnetic field for 15 minutes, and absorbing supernatant after carboxyl magnetic beads are completely settled; adding a phosphate buffer solution which is 5 times of the volume of the carboxyl magnetic beads, and uniformly mixing; step 3, placing in a magnetic field, and after all the magnetic beads settle, absorbing supernatant liquid; 4, repeating the step 2 and the step 3 for three times to fully clean the carboxyl magnetic beads; and 5, fixing the volume of the carboxyl magnetic bead solution t