In-vitro evaluation method for moisturizing effect of material

The invention provides an in-vitro evaluation method for the moisturizing effect of a material, which comprises the following steps: constructing a cell model by using human immortal epidermal cells (HaCaT cells), acting a tested material on the cell model, and evaluating the moisturizing effect of the tested material by characterizing the content variation of moisturizing factors in the cell model caused by the action of the tested material on the cell model. Wherein the moisturizing factor comprises at least one of pyrrolidone carboxylic acid (PCA) and urocanic acid (UCA). The change of the PCA content and the UCA content in the HaCaT cells can accurately reflect the moisturizing effect condition of the material, so that the in-vitro evaluation method for the moisturizing effect of the material provided by the invention can accurately and reliably evaluate the moisturizing effect of the tested material acting on the human skin. 本发明提供一种物料保湿功效的体外评价方法，以人永生表皮细胞(HaCaT细胞)构建细胞模型，将参试物料作用于细胞模型，通过表征参试物料作用于细胞模型所引起细胞模型