Method for detecting five steroid hormones in blood sample by ultra-high performance liquid chromatography-tandem mass spectrometry, kit and application

The invention discloses a method for detecting five steroid hormones in a blood sample by an ultra-high performance liquid chromatography-tandem mass spectrometry technology, a kit and application, and relates to the technical field of steroid hormone detection and analysis. The method for detecting steroid hormones in a blood sample comprises the following steps: S1, carrying out pretreatment on the blood sample, namely uniformly mixing the blood sample with a precipitant containing an internal standard, centrifuging, taking supernate, drying, and redissolving with a resolvent to obtain a to-be-detected solution; the internal standard is a standard substance of the steroid hormone labeled by isotope; s2, detecting the liquid to be detected by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry technology; the steroid hormones comprise testosterone, androstenedione, dihydrotestosterone, 17 alpha-hydroxyprogesterone and dehydroepiandrosterone sulfate. The method can accurately det