Plant MRM wide-target metabonomics detection method

The invention relates to G01N, in particular to a plant MRM wide-target metabonomics detection method. Comprising the following steps: mixing a sample with an internal standard, carrying out ultrasonic treatment and centrifugation, and carrying out UHPLC-MS detection on a supernatant. The invention...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: FAN YANQUN, ZHENG SIYAN, LOU GEER, LIU YU
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to G01N, in particular to a plant MRM wide-target metabonomics detection method. Comprising the following steps: mixing a sample with an internal standard, carrying out ultrasonic treatment and centrifugation, and carrying out UHPLC-MS detection on a supernatant. The invention provides a plant MRM wide-target metabonomics detection method which can be used for detecting various metabolites of different plants. The detected target metabolites can cover flavonoids, polyphenols, alkaloids, terpenes, quinones, steroids, phenylpropanoids, vitamins, organic acids, nucleotides, sugars, alcohols, lipids, aromatics, coumarins, lignans, carboxylic acids, organic oxygen, plant hormones, tryptamine, indole, pyridine, imidazoles and the like. High-flux, high-sensitivity and high-resolution qualitative and quantitative detection of different target metabolites can be met, QC sample correlation and response stability in an internal standard are improved, and instrument and data quality of the detection