Enzymatic pretreatment process of deodorized distillate and application of deodorized distillate in purification of natural vitamin E

The invention relates to the technical field of biochemical engineering, in particular to an enzymatic pretreatment process of deodorized distillate and application of the deodorized distillate in purification of natural vitamin E. The enzymatic pretreatment process comprises the following steps: firstly, adding lipase into a deodorized distillate, feeding a certain mass of methanol through a constant flow pump, after the acid value of a system is reduced to 6-8mgKOH/g, carrying out vacuum water removal to push the reaction to proceed forwards, and then continuously feeding methanol to carry out methyl esterification reaction until the acid value of the system is reduced to be lower than 3mgKOH/g. The method has the advantages of mild reaction conditions, low requirements on equipment conditions, good acid resistance of the used lipase, no need of carrier immobilization, great reduction of the reaction cost, simple operation steps, realization of the purification pretreatment standard of the natural vitamin E