Screening system of pasteurella multocida gene deletion mutant strain and application thereof

The invention relates to a screening system of a pasteurella multocida gene deletion mutant strain and application of the screening system, and belongs to the technical field of biology.Firstly, a recombinant expression vector containing a pasteurella multocida glgB and opa gene knockout box and a lacZ gene is constructed, the recombinant expression vector is introduced into a modified target strain C48-1, and a mutant strain C48-1 is obtained; mutant strains can be conveniently and efficiently screened by utilizing a temperature sensitive system and blue-white spot screening. According to the method, successful knockout of the pasteurella multocida gene is realized, the time and energy for screening mutant strains can be greatly reduced, and the mutant strains can be efficiently and quickly screened. 本发明涉及多杀性巴氏杆菌基因缺失突变株的筛选系统及其应用，属于生物技术领域，本发明首先构建含有多杀性巴氏杆菌glgB和opa基因敲除盒与lacZ基因的重组表达载体，将所述重组表达载体导进改造目的菌株C48-1株，分别通过利用温度敏感系统与蓝白斑筛选，方便高效地筛选出突变株。该方法实现了多杀性巴氏杆菌基因的成功敲除，并能够大幅度地减少了筛选突变株的时间和精力，达到高效快速地筛选突变株。