Cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 as well as coding gene and application thereof

The invention discloses a cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 as well as a coding gene and application of the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343. The amino acid sequence of the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 is as shown in SE...

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Hauptverfasser:	LV DONGHAI, ZHU WEIYUN, CHENG YANFEN, MENG ZHENXIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 as well as a coding gene and application of the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343. The amino acid sequence of the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 is as shown in SEQ ID No.3, and the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343 is obtained by mutating cysteine at the 343th site of a cellulase-xylanase bifunctional enzyme with the amino acid sequence as SEQ ID No.1 into serine. The mutant has good capability of hydrolyzing natural lignocellulose, and can be used for preparing the cellulase-xylanase bifunctional enzyme mutant CelXyn2-S343. The method has theoretical guiding significance in the aspects of improving the digestibility of feed fibers and improving the production performance of animals. 本发明公开了一种纤维素酶-木聚糖酶双功能酶突变体CelXyn2-S343及其编码基因和应用。所述纤维素酶-木聚糖酶双功能酶突变体CelXyn2-S343的氨基酸序列如SEQ ID No.3所示，是由氨基酸序列为SEQ ID No.1的纤维素酶-木聚糖酶双功能酶的第343位的半胱氨酸突变成丝氨酸获得的，该突变体具有良好的水解天然木质纤维