Flavonoid glycosyl transferase as well as coding gene and application thereof

The invention belongs to the technical field of gene engineering and enzyme engineering, and relates to stevia rebaudiana sourced flavonoid glycosyl transferase as well as a coding gene and application thereof. The flavonoid glycosyl transferase disclosed by the invention is derived from stevia reba...

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Hauptverfasser: XU LIANGPING, YIN HENG, ZHU LIPING, CHEN KECAI, WANG YU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene engineering and enzyme engineering, and relates to stevia rebaudiana sourced flavonoid glycosyl transferase as well as a coding gene and application thereof. The flavonoid glycosyl transferase disclosed by the invention is derived from stevia rebaudiana, and the amino acid sequence of the flavonoid glycosyl transferase is The nucleotide sequence of the gene is as shown in SEQ ID NO. 2. The flavonoid glycosyl transferase SrUGT72B1 is a 3-, 5-, 7-O glycosyl transferase capable of catalyzing glycosylation of flavonoid compounds, has broad-spectrum catalytic activity, can catalyze glycosylation of flavonol compounds, flavonoid compounds, flavanone compounds and dihydroxy chalcone compounds, and can catalyze glycosylation of flavonol (quercetin, kaempferol and quercetin-3-O-glucoside), flavones (apigenin, apigenin, apigenin, apigenin, apigenin, apigenin, apigenin and apigenin), apigenin, apigenin, apigenin, apigenin, apigenin, apigenin, apigenin, apigenin and ap