Carbonyl reductase mutant and application thereof

The invention discloses a carbonyl reductase mutant and application thereof. The mutation sites of the carbonyl reductase mutant comprise the 88th site, the 142 site, the 190th site and the 193th site of an amino acid sequence shown as SEQ ID NO: 1. Compared with wild type carbonyl reductase, the carbonyl reductase mutant disclosed by the invention has higher enzyme activity. Wherein the enzymatic activity of partial carbonyl reductase mutants is 50 times of that of wild carbonyl. According to the carbonyl reductase mutant, in a liquid reaction system, in the presence of coenzyme, a compound shown in a formula I can be subjected to a reduction reaction shown in the following formula, and the conversion rate is gt; the chiral ee value is gt; the chiral de value is gt; the compound shown as the formula I I is prepared. 本发明公开了一种羰基还原酶突变体及其应用。本发明的羰基还原酶突变体的突变位点包括如SEQ ID NO:1所示的氨基酸序列的第88位、第142位、第190位和第193位。本发明的羰基还原酶突变体相对于野生型羰基还原酶具有更高的酶活性。其中部分羰基还原酶突变体的酶活性是野生型羰基的50倍。本发明的羰基还原酶突变体在液态反应体系中，在辅酶存在下，能使如式I所示化合物进行如下式所示的还原反应，并