Preparation method of corydalis saxicola bunting leaf protoplast

The invention discloses a preparation method of corydalis saxicola bunting leaf protoplast, which comprises the following steps: 1) cutting mature corydalis saxicola bunting leaves into shreds, placing the shreds in an enzymatic hydrolysate for enzymolysis treatment, the mass volume ratio of the corydalis saxicola bunting shreds to the enzymatic hydrolysate is 1: (8-12) g/mL, and the enzymatic hydrolysate contains 2%-3% of cellulase R-10 and 0.25%-1% of macerozyme R-10; (2) filtering the mixed solution subjected to enzymolysis by using a sterile filter screen to remove plant tissues and cell clusters which are not subjected to enzymolysis completely, taking filtrate, centrifuging the filtrate for 2-5 minutes under the condition of 800-1400 r/min, and removing supernate to obtain a precipitate; and 3) resuspending the precipitate with CPW washing liquor, centrifuging, and collecting cell precipitate to obtain the corydalis saxicola bunting leaf protoplast. The corydalis saxicola bunting leaf protoplast prepare