Recombinant saccharomyces cerevisiae for producing cannabinoid phenolic acid as well as construction method and application of recombinant saccharomyces cerevisiae

The invention relates to recombinant saccharomyces cerevisiae for producing cannabinoid phenolic acid, the recombinant saccharomyces cerevisiae is prepared from basic saccharomyces cerevisiae, and the following endogenous genes of the basic saccharomyces cerevisiae are over-expressed, down-regulated or knocked out: pep4, gal80, gal4 and fast 1; in some embodiments, exogenous genes encoding a tetraketone synthase (Cs.TKS) gene, an olive toluate cyclase (Cs.OAC) gene, a geranyl pyrophosphate: olivate geranyl transferase (Cs.PT4n) gene, and a variant acetyl coenzyme synthase (Se. ACS) gene are overexpressed. The saccharomyces cerevisiae capable of synthesizing the CBGA is used as an original strain, Cs.TKS, Cs.OAC, Cs.PT4 and Se.ACS are selectively over-expressed, gal80 is selectively knocked out and gal4 is over-expressed, pep4 is selectively knocked out, Cs.OAC which passes through or does not pass through linker fusion is selectively over-expressed, Cs.PT4n enzyme is positioned to a plasma membrane through or