Modified centromere protein as well as preparation method, biological material and application of modified centromere protein

The invention provides a modified centromere protein as well as a preparation method, a biological material and application of the modified centromere protein, and relates to the technical field of protein engineering. The modified protein of the centromere protein sequentially comprises a fragment B5, a fragment B6, a fragment B2 and a fragment A1 from a nitrogen terminal to a carbon terminal, wherein the amino acid sequences of the fragment B5, the fragment B6, the fragment B2 and the fragment A1 are respectively shown as Seq 1-4. The modified protein of the centromere protein contains a CENP-A antigen and a CENP-B antigen at the same time, and the problem that in the prior art, the sensitivity and specificity are poor when one antigen is independently used for detecting a centromere antibody is solved. 本发明提供了一种着丝粒蛋白改构蛋白及其制备方法、生物材料和应用，涉及蛋白工程的技术领域。该着丝粒蛋白改构蛋白由氮端至碳端依次包含片段B5、片段B6、片段B2和片段A1，片段B5、片段B6、片段B2和片段A1氨基酸序列分别如Seq_1～4所示。该着丝粒蛋白改构蛋白同时含有CENP-A抗原和CENP-B抗原，缓解了现有技术中存在的单独使用一种抗原检测着丝粒抗体的灵敏度和特异性不佳的问题。