Polygonatum cyrtonema tissue culture method

The invention relates to the field of tissue culture of medicinal plants, and particularly discloses a tissue culture method of polygonatum cyrtonema, which is characterized in that seeds obtained after immature seeds of polygonatum cyrtonema are subjected to refrigeration and mechanical damage treatment are used as explants for tissue culture. According to the method, immature seeds are used as materials, refrigeration is performed to break dormancy, mechanical damage treatment is performed to promote embryo germination so as to obtain explants, a set of tissue culture system for rapidly and massively propagating polygonatum cyrtonema plantlets is subsequently constructed for the explants, industrialized seedling production of polygonatum cyrtonema can be realized, and promotion of the molecular breeding age is accelerated. 本发明涉及药用植物组织培养领域，具体公开了一种多花黄精组织培养方法，为以多花黄精未成熟种子经冷藏和机械损伤处理后得到的种子作为外植体进行组织培养。本发明的方法使用未成熟种子为材料，冷藏打破休眠，机械损伤处理促进种胚萌发得到外植体，后续针对外植体构建了一套快速、大量繁殖出多花黄精小苗的组培体系，可实现多花黄精的工厂化育苗，加快分子育种时代的推进。