Haploid inducing compositions and methods of use thereof

Provided herein are methods of inducing haploid induction in a plant using a mutated potato glycoprotein-like phospholipase II [alpha] ("pPLAII [alpha]", renamed herein as MATRILINEAL), cloning pPLAII [alpha] to induce haploid induction in a plant, and genetically engineering a plant to comprise the mutated pPLAII [alpha]. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to a plant during pollination in order to induce haploid production. Further provided are methods of chemically treating a plant during pollination to induce haploids while also reducing embryo abortion and increasing seed setting. 本文提供了使用突变的马铃薯糖蛋白样磷脂酶IIα("pPLAIIα"，这里重新命名为MATRILINEAL)在植物中诱导单倍体诱导，克隆pPLAIIα以在植物中诱导单倍体诱导，和遗传工程化植物以包含突变的pPLAIIα的方法。还提供了在授粉期间向植物施用局部和喷雾化学物质、脂质和RNAi分子以便诱导单倍体产生的方法。进一步提供了在授粉期间化学处理植物以诱导单倍体同时还减少胚败育和增加结籽的方法。