Flow cell and preparation method thereof

An exemplary flow cell is disclosed, the flow cell comprising a multi-layer stack, the multi-layer stack comprising a transparent susceptor carrier; a patterned sacrificial layer over the transparent base carrier; and a transparent layer over the patterned sacrificial layer. The flow cell further includes a first functionalization layer and a second functionalization layer over different portions of the transparent layer, wherein at least one of the first functionalization layer and the second functionalization layer is aligned with a pattern of the patterned sacrificial layer; and a first primer set and a second primer set attached to the first functionalized layer and the second functionalized layer, respectively. 本发明公开了一种示例性流通池，所述流通池包括多层叠堆，所述多层叠堆包括透明基座载体；所述透明基座载体上方的图案化牺牲层；以及所述图案化牺牲层上方的透明层。所述流通池还包括所述透明层的不同部分上方的第一官能化层和第二官能化层，其中所述第一官能化层和所述第二官能化层中的至少一者与所述图案化牺牲层的图案对准；以及分别附接到所述第一官能化层和所述第二官能化层的第一引物组和第二引物组。