Method for simultaneously detecting six components of Qingkailing preparation
The invention discloses a method for simultaneously detecting six components of a Qingkailing preparation. The method comprises the following steps: preparing a test solution and a reference solution; respectively adding the test solution and the reference solution into a thin-layer plate; taking et...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for simultaneously detecting six components of a Qingkailing preparation. The method comprises the following steps: preparing a test solution and a reference solution; respectively adding the test solution and the reference solution into a thin-layer plate; taking ethyl acetate, acetone, formic acid and water in a volume ratio of (22-28): (12-18): (1-5): (2-7) as a developing solvent, and fully reacting with the thin-layer plate obtained in the previous step; and the thin-layer plate after the reaction is subjected to color development under ultraviolet light of 250-260 nm. The method can qualitatively detect six traditional Chinese medicine effective components including geniposide, chlorogenic acid, baicalin, galuteolin, isochlorogenic acid A and isochlorogenic acid C at the same time, is simple and rapid to operate, clear in color development result and easy to interpret, has good specificity and reproducibility, realizes quality control of a compound traditional Chinese me |
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