Pretreatment method for efficient detection of beta receptor stimulant in animal-derived food and application

The invention discloses a pretreatment method for efficiently detecting a beta receptor stimulant in animal-derived food, which belongs to the field of food safety and comprises the following steps: weighing 2.00 g of sample, adding 8 mL of 0.2 moL/L sodium acetate buffer solution into a 50 mL centrifugal tube; adding 100 [mu] L of a 100 ng/mL mixed internal standard solution; adding 50 [mu] L of beta glucuronidase hydrochloride/aryl sulfatase, uniformly mixing, and carrying out enzymolysis overnight at 37 DEG C; according to the pretreatment method for efficient detection of the beta receptor stimulant in the animal-derived food and the application, the concentrated perchloric acid is used for precipitating protein, and compared with dilute perchloric acid, the use amount is reduced, the total volume of liquid is reduced, and follow-up treatment is facilitated; a CO32-HCO3-buffer system (PKa = 10.2) is introduced in the PH adjusting process, and after related reagents are added according to the PH adjusting