Preparation method of multi-single-cell miRNA sequencing library based on digital micro-fluidic chip

The invention discloses a method for preparing a plurality of single-cell miRNA sequencing libraries based on a digital micro-fluidic chip, and the digital micro-fluidic chip is provided with at least one liquid storage region, at least one liquid drop generation channel, a single-cell capture region and a plurality of library reaction regions which are sequentially communicated and provided with a super-hydrophobic surface, the at least one liquid storage area, the at least one liquid drop generation channel, the single cell capture area and the plurality of library reaction areas are all provided with electrodes electrically connected with a driving electrode of the digital micro-fluidic chip, the single cell capture area is provided with a plurality of hydrophilic parts which are uniformly distributed, and each hydrophilic part is correspondingly communicated with one library reaction area. According to the invention, efficient, automatic and selective simultaneous separation of a plurality of single cells