Method for constructing renal fibrosis by Gas5 gene knockout mouse
The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a metho...
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| creator | ZHOU JIAYUE XIANG XIAOJUN YE QINGQING KO SANG-BOK SHOU QIYANG HU SHOUCI ZHANG XIANG CHEN HONGBO |
| description | The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a method for constructing renal fibrosis by using the Gas5 gene knockout mouse. According to the invention, the CRISPR-Cas9 technology is used for constructing lncRNA Gas5 knockout homozygote (Gas5-/-) and heterozygote (Gas5 +/-) mice, so that an ideal animal model basis is provided for researching the effect of lncRNA Gas5 in renal fibrosis and researching the mechanism of lncRNA Gas5.
本发明属于生物领域，具体涉及Gas5基因敲除小鼠构建肾纤维化模型的方法。本发明提供了用于Gas5基因敲除小鼠构建肾纤维化的Cas9/sgRNA靶点，本发明还提供了Gas5基因敲除小鼠构建肾纤维化的方法。本发明运用CRISPR-Cas9技术构建lncRNA Gas5敲除的纯合子(Gas5-/-)以及杂合子(Gas5+/-)小鼠，从而为探究lncRNA Gas5在肾纤维化中的作用及其机制研究提供了理想的动物模型基础。 |
| format | Patent |
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本发明属于生物领域，具体涉及Gas5基因敲除小鼠构建肾纤维化模型的方法。本发明提供了用于Gas5基因敲除小鼠构建肾纤维化的Cas9/sgRNA靶点，本发明还提供了Gas5基因敲除小鼠构建肾纤维化的方法。本发明运用CRISPR-Cas9技术构建lncRNA Gas5敲除的纯合子(Gas5-/-)以及杂合子(Gas5+/-)小鼠，从而为探究lncRNA Gas5在肾纤维化中的作用及其机制研究提供了理想的动物模型基础。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CARE OF BIRDS, FISHES, INSECTS ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW BREEDS OF ANIMALS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230404&DB=EPODOC&CC=CN&NR=115896174A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230404&DB=EPODOC&CC=CN&NR=115896174A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHOU JIAYUE</creatorcontrib><creatorcontrib>XIANG XIAOJUN</creatorcontrib><creatorcontrib>YE QINGQING</creatorcontrib><creatorcontrib>KO SANG-BOK</creatorcontrib><creatorcontrib>SHOU QIYANG</creatorcontrib><creatorcontrib>HU SHOUCI</creatorcontrib><creatorcontrib>ZHANG XIANG</creatorcontrib><creatorcontrib>CHEN HONGBO</creatorcontrib><title>Method for constructing renal fibrosis by Gas5 gene knockout mouse</title><description>The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a method for constructing renal fibrosis by using the Gas5 gene knockout mouse. According to the invention, the CRISPR-Cas9 technology is used for constructing lncRNA Gas5 knockout homozygote (Gas5-/-) and heterozygote (Gas5 +/-) mice, so that an ideal animal model basis is provided for researching the effect of lncRNA Gas5 in renal fibrosis and researching the mechanism of lncRNA Gas5.
本发明属于生物领域，具体涉及Gas5基因敲除小鼠构建肾纤维化模型的方法。本发明提供了用于Gas5基因敲除小鼠构建肾纤维化的Cas9/sgRNA靶点，本发明还提供了Gas5基因敲除小鼠构建肾纤维化的方法。本发明运用CRISPR-Cas9技术构建lncRNA Gas5敲除的纯合子(Gas5-/-)以及杂合子(Gas5+/-)小鼠，从而为探究lncRNA Gas5在肾纤维化中的作用及其机制研究提供了理想的动物模型基础。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CARE OF BIRDS, FISHES, INSECTS</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW BREEDS OF ANIMALS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDyTS3JyE9RSMsvUkjOzysuKSpNLsnMS1coSs1LzFFIy0wqyi_OLFZIqlRwTyw2VUhPzUtVyM7LT87OLy1RyM0vLU7lYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyUA9JfHOfoaGphaWZobmJo7GxKgBADQ6Mfw</recordid><startdate>20230404</startdate><enddate>20230404</enddate><creator>ZHOU JIAYUE</creator><creator>XIANG XIAOJUN</creator><creator>YE QINGQING</creator><creator>KO SANG-BOK</creator><creator>SHOU QIYANG</creator><creator>HU SHOUCI</creator><creator>ZHANG XIANG</creator><creator>CHEN HONGBO</creator><scope>EVB</scope></search><sort><creationdate>20230404</creationdate><title>Method for constructing renal fibrosis by Gas5 gene knockout mouse</title><author>ZHOU JIAYUE ; XIANG XIAOJUN ; YE QINGQING ; KO SANG-BOK ; SHOU QIYANG ; HU SHOUCI ; ZHANG XIANG ; CHEN HONGBO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115896174A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CARE OF BIRDS, FISHES, INSECTS</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW BREEDS OF ANIMALS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHOU JIAYUE</creatorcontrib><creatorcontrib>XIANG XIAOJUN</creatorcontrib><creatorcontrib>YE QINGQING</creatorcontrib><creatorcontrib>KO SANG-BOK</creatorcontrib><creatorcontrib>SHOU QIYANG</creatorcontrib><creatorcontrib>HU SHOUCI</creatorcontrib><creatorcontrib>ZHANG XIANG</creatorcontrib><creatorcontrib>CHEN HONGBO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHOU JIAYUE</au><au>XIANG XIAOJUN</au><au>YE QINGQING</au><au>KO SANG-BOK</au><au>SHOU QIYANG</au><au>HU SHOUCI</au><au>ZHANG XIANG</au><au>CHEN HONGBO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for constructing renal fibrosis by Gas5 gene knockout mouse</title><date>2023-04-04</date><risdate>2023</risdate><abstract>The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a method for constructing renal fibrosis by using the Gas5 gene knockout mouse. According to the invention, the CRISPR-Cas9 technology is used for constructing lncRNA Gas5 knockout homozygote (Gas5-/-) and heterozygote (Gas5 +/-) mice, so that an ideal animal model basis is provided for researching the effect of lncRNA Gas5 in renal fibrosis and researching the mechanism of lncRNA Gas5.
本发明属于生物领域，具体涉及Gas5基因敲除小鼠构建肾纤维化模型的方法。本发明提供了用于Gas5基因敲除小鼠构建肾纤维化的Cas9/sgRNA靶点，本发明还提供了Gas5基因敲除小鼠构建肾纤维化的方法。本发明运用CRISPR-Cas9技术构建lncRNA Gas5敲除的纯合子(Gas5-/-)以及杂合子(Gas5+/-)小鼠，从而为探究lncRNA Gas5在肾纤维化中的作用及其机制研究提供了理想的动物模型基础。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE |
| title | Method for constructing renal fibrosis by Gas5 gene knockout mouse |
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