Method for constructing renal fibrosis by Gas5 gene knockout mouse

Method for constructing renal fibrosis by Gas5 gene knockout mouse

The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a metho...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZHOU JIAYUE, XIANG XIAOJUN, YE QINGQING, KO SANG-BOK, SHOU QIYANG, HU SHOUCI, ZHANG XIANG, CHEN HONGBO
Format: Patent
Sprache:chi ; eng
Schlagworte:
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention belongs to the field of biology, and particularly relates to a method for constructing a renal fibrosis model by a Gas5 gene knockout mouse. The invention provides a Cas9/sgRNA target spot for constructing renal fibrosis by using a Gas5 gene knockout mouse, and further provides a method for constructing renal fibrosis by using the Gas5 gene knockout mouse. According to the invention, the CRISPR-Cas9 technology is used for constructing lncRNA Gas5 knockout homozygote (Gas5-/-) and heterozygote (Gas5 +/-) mice, so that an ideal animal model basis is provided for researching the effect of lncRNA Gas5 in renal fibrosis and researching the mechanism of lncRNA Gas5. 本发明属于生物领域，具体涉及Gas5基因敲除小鼠构建肾纤维化模型的方法。本发明提供了用于Gas5基因敲除小鼠构建肾纤维化的Cas9/sgRNA靶点，本发明还提供了Gas5基因敲除小鼠构建肾纤维化的方法。本发明运用CRISPR-Cas9技术构建lncRNA Gas5敲除的纯合子(Gas5-/-)以及杂合子(Gas5+/-)小鼠，从而为探究lncRNA Gas5在肾纤维化中的作用及其机制研究提供了理想的动物模型基础。