SgRNA for targeted knockout of human TMEM121 gene, method for constructing gene-deleted cell strain and application

The invention discloses sgRNA for targeted knockout of a human TMEM121 gene, a method for constructing a TMEM121 gene deletion cell strain and application, and belongs to the technical field of biological medicine. The method comprises the following steps: constructing a pX459-TMEM121 recombinant plasmid by virtue of a Cas9 system, transfecting an HEK293T cell by virtue of the Cas9 recombinant plasmid, screening by virtue of Puromycin, and combining a limited dilution method with HEK293T screening, so as to obtain the stable cell strain HEK293T cell and Huh7 cell of which the TMEM121 gene is knocked out. DNA (deoxyribonucleic acid) detection shows that the TMEM121 gene of the gene knockout cell strain is deleted. In addition, the migration and invasion capacities of the cell strain with the TMEM121 gene knocked out are obviously reduced. The result shows that after the TMEM121 gene is successfully knocked out by using the Cas9 system, the malignant transformation of liver cancer cells can be hindered. 本发明公开了靶