Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process
The invention relates to a method for improving the dynamic loading capacity of ion exchange chromatography in the purification process of plasmid DNA, and spermine or spermidine is used as a compacting agent when the plasmid DNA is purified by ion exchange chromatography. According to the method, s...
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| creator | YANG WEI LIANG HUA XIAO YAO REN KEYUN YE WEI |
| description | The invention relates to a method for improving the dynamic loading capacity of ion exchange chromatography in the purification process of plasmid DNA, and spermine or spermidine is used as a compacting agent when the plasmid DNA is purified by ion exchange chromatography. According to the method, spermine or spermidine is used as a compacting agent in the plasmid DNA ion-exchange chromatography process, so that the ion-exchange chromatography dynamic loading capacity in the plasmid DNA purification process can be remarkably improved, the chromatography behavior in the plasmid DNA chromatography process is more uniform, a sharper chromatography peak is formed, the use amount of the filler can be reduced, and the filler utilization rate can be improved.
本发明涉及一种提高质粒DNA纯化过程中的离子交换层析动态载量的方法，在质粒DNA采用离子交换层析提纯时，以精胺或亚精胺作为压实剂。本发明在质粒DNA离子交换层析过程中以精胺或亚精胺作为压实剂，能够显著提高质粒DNA纯化过程中的离子交换层析动态载量，使质粒DNA的层析过程中层析行为更加均匀、形成更尖锐的层析峰，可降低填料的使用量，提高填料利用率。 |
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本发明涉及一种提高质粒DNA纯化过程中的离子交换层析动态载量的方法，在质粒DNA采用离子交换层析提纯时，以精胺或亚精胺作为压实剂。本发明在质粒DNA离子交换层析过程中以精胺或亚精胺作为压实剂，能够显著提高质粒DNA纯化过程中的离子交换层析动态载量，使质粒DNA的层析过程中层析行为更加均匀、形成更尖锐的层析峰，可降低填料的使用量，提高填料利用率。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230404&DB=EPODOC&CC=CN&NR=115896097A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230404&DB=EPODOC&CC=CN&NR=115896097A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANG WEI</creatorcontrib><creatorcontrib>LIANG HUA</creatorcontrib><creatorcontrib>XIAO YAO</creatorcontrib><creatorcontrib>REN KEYUN</creatorcontrib><creatorcontrib>YE WEI</creatorcontrib><title>Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process</title><description>The invention relates to a method for improving the dynamic loading capacity of ion exchange chromatography in the purification process of plasmid DNA, and spermine or spermidine is used as a compacting agent when the plasmid DNA is purified by ion exchange chromatography. According to the method, spermine or spermidine is used as a compacting agent in the plasmid DNA ion-exchange chromatography process, so that the ion-exchange chromatography dynamic loading capacity in the plasmid DNA purification process can be remarkably improved, the chromatography behavior in the plasmid DNA chromatography process is more uniform, a sharper chromatography peak is formed, the use amount of the filler can be reduced, and the filler utilization rate can be improved.
本发明涉及一种提高质粒DNA纯化过程中的离子交换层析动态载量的方法，在质粒DNA采用离子交换层析提纯时，以精胺或亚精胺作为压实剂。本发明在质粒DNA离子交换层析过程中以精胺或亚精胺作为压实剂，能够显著提高质粒DNA纯化过程中的离子交换层析动态载量，使质粒DNA的层析过程中层析行为更加均匀、形成更尖锐的层析峰，可降低填料的使用量，提高填料利用率。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzLEKwjAQgOEuDqK-w_kAgkXUdixVcbGTezmSa3LQ5EISxb69Cj6A07_8fPMCb5StaBgkArsQ5cneAIsHeimL3hAoG8VhFhMx2An05NGxglFQA3sIIybHGk5dA-EReWCF-Qt8MEUpLYvZgGOi1a-LYn0539vrhoL0lAIq8pT7tivLfVUftvWx2f3zvAEqlz39</recordid><startdate>20230404</startdate><enddate>20230404</enddate><creator>YANG WEI</creator><creator>LIANG HUA</creator><creator>XIAO YAO</creator><creator>REN KEYUN</creator><creator>YE WEI</creator><scope>EVB</scope></search><sort><creationdate>20230404</creationdate><title>Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process</title><author>YANG WEI ; LIANG HUA ; XIAO YAO ; REN KEYUN ; YE WEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115896097A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YANG WEI</creatorcontrib><creatorcontrib>LIANG HUA</creatorcontrib><creatorcontrib>XIAO YAO</creatorcontrib><creatorcontrib>REN KEYUN</creatorcontrib><creatorcontrib>YE WEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANG WEI</au><au>LIANG HUA</au><au>XIAO YAO</au><au>REN KEYUN</au><au>YE WEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process</title><date>2023-04-04</date><risdate>2023</risdate><abstract>The invention relates to a method for improving the dynamic loading capacity of ion exchange chromatography in the purification process of plasmid DNA, and spermine or spermidine is used as a compacting agent when the plasmid DNA is purified by ion exchange chromatography. According to the method, spermine or spermidine is used as a compacting agent in the plasmid DNA ion-exchange chromatography process, so that the ion-exchange chromatography dynamic loading capacity in the plasmid DNA purification process can be remarkably improved, the chromatography behavior in the plasmid DNA chromatography process is more uniform, a sharper chromatography peak is formed, the use amount of the filler can be reduced, and the filler utilization rate can be improved.
本发明涉及一种提高质粒DNA纯化过程中的离子交换层析动态载量的方法，在质粒DNA采用离子交换层析提纯时，以精胺或亚精胺作为压实剂。本发明在质粒DNA离子交换层析过程中以精胺或亚精胺作为压实剂，能够显著提高质粒DNA纯化过程中的离子交换层析动态载量，使质粒DNA的层析过程中层析行为更加均匀、形成更尖锐的层析峰，可降低填料的使用量，提高填料利用率。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process |
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