Method for improving ion exchange chromatography dynamic load in plasmid DNA purification process

The invention relates to a method for improving the dynamic loading capacity of ion exchange chromatography in the purification process of plasmid DNA, and spermine or spermidine is used as a compacting agent when the plasmid DNA is purified by ion exchange chromatography. According to the method, spermine or spermidine is used as a compacting agent in the plasmid DNA ion-exchange chromatography process, so that the ion-exchange chromatography dynamic loading capacity in the plasmid DNA purification process can be remarkably improved, the chromatography behavior in the plasmid DNA chromatography process is more uniform, a sharper chromatography peak is formed, the use amount of the filler can be reduced, and the filler utilization rate can be improved. 本发明涉及一种提高质粒DNA纯化过程中的离子交换层析动态载量的方法，在质粒DNA采用离子交换层析提纯时，以精胺或亚精胺作为压实剂。本发明在质粒DNA离子交换层析过程中以精胺或亚精胺作为压实剂，能够显著提高质粒DNA纯化过程中的离子交换层析动态载量，使质粒DNA的层析过程中层析行为更加均匀、形成更尖锐的层析峰，可降低填料的使用量，提高填料利用率。