Cellobiose dehydrogenase gene, vector, recombinant bacterium and application thereof

The invention discloses a cellobiose dehydrogenase gene, a carrier, recombinant bacteria and application thereof, grifola frondosa is used as a cellobiose dehydrogenase gene donor, and the grifola frondosa cellobiose dehydrogenase gene is obtained through PCR (polymerase chain reaction) amplification. And a recombinant expression vector is constructed. Aspergillus niger C112 is used as a gene expression host, the recombinant expression vector is introduced into the aspergillus niger through a PEG-mediated protoplast method, the cellobiose dehydrogenase gene is successfully expressed in the aspergillus niger, and the recombinant grifola frondosa cellobiose dehydrogenase can improve the activity of cellulase and manganese peroxidase of the aspergillus niger C112. The addition amount of the laccase, the cellobiose dehydrogenase and the cellobiase in a system for synergistically degrading the poplar fibers by multiple enzymes is optimized, and an optimal enzymolysis combination system of the cellulase, the laccas