Vero cell strain applied to virus amplification as well as construction method and application of Vero cell strain

The invention discloses a Vero cell strain applied to virus amplification as well as a construction method and application of the Vero cell strain. The Vero cell strain is formed by knocking out two gene segments of STAT1 and EMX2 in a genome of the Vero cell strain. Compared with the prior art, both of STAT1 and EMX2 in the Vero cell strain are knocked out, so that when the Vero cell strain is applied to amplification of the porcine epidemic diarrhea virus and the like, the titer of the virus can be remarkably improved, and the Vero cell strain has a wide application prospect in preparation of live virus vaccines and inactivated vaccines. 本申请公开了一种应用于病毒扩增的Vero细胞株、其构建方法及应用。所述Vero细胞株是通过将Vero细胞株基因组中STAT1和EMX2两个基因片段敲除后形成。与现有技术相比，本申请通过将Vero细胞株中的STAT1和EMX2中的两者敲除，使其在应用于猪流行性腹泻病毒等的扩增时，能够显著提高病毒的滴度，在病毒活疫苗和灭活疫苗制备中有广阔应用前景。