Bionic recombinant V-type mussel mucin as well as preparation method and application thereof

The invention discloses a bionic recombinant V-type mussel mucin as well as a preparation method and application thereof. The recombinant V-type mussel mucoprotein is formed by fusing Mfp-5 of natural mussels and protein forming a mussel byssus fiber skeleton. In the preparation process, a fusion gene and a tyrosinase gene are co-expressed, and the expressed tyrosinase is used for performing dopa modification on tyrosine residues in the recombinant mussel mucin, so that the recombinant V-type mussel mucin with biological activity is obtained, and the recombinant V-type mussel mucin is high in yield, high in purity and low in endotoxin content. The application requirements in the fields of medicines, medical instruments and cosmetics are completely met. 本发明公开了一种仿生重组V型贻贝粘蛋白及其制备方法和应用。所述的重组V型贻贝粘蛋白是由天然贻贝的Mfp-5和构成贻贝足丝纤维骨架的蛋白融合组成。在制备过程中，将融合基因与酪氨酸酶基因共表达，并利用表达的酪氨酸酶对重组贻贝粘蛋白中的酪氨酸残基进行多巴修饰，从而获得具有生物活性的重组V型贻贝粘蛋白，并且产量高、纯度高、内毒素含量低，完全满足药品、医疗器械及化妆品领域的应用需求。