CIT fluorophore of 1-furan and thienyl-2-alkenyl-1-ketone containing nitrogen mustard as well as preparation method and application of CIT fluorophore

The invention discloses a nitrogen mustard-containing 1-furan, thienyl-2-alkenyl-1-ketone CIT fluorophore and a preparation method and application thereof, and belongs to the field of medical intermediates, the nitrogen mustard-containing 1-furan, thienyl-2-alkenyl-1-ketone CIT fluorophore has a molecular structure as shown in formula (I), in the formula (I), X is O or S; r is one or more of H, CH3 and Cl. The preparation method comprises the following steps: uniformly mixing 4-[bis (beta-chloroethyl) amino] benzaldehyde and an acetyl-containing furan derivative or an acetyl-containing thiophene derivative in a solvent, and then carrying out condensation reaction under an alkali catalysis condition to prepare the compound shown in the formula (I). Meanwhile, the invention discloses application of the ICT fluorophore in cell fluorescence imaging and preparation of antitumor drugs. The invention also discloses an antitumor drug comprising the ICT fluorophore. The obtained ICT fluorophore has strong pharmacologi