Method for detecting steroid hormone in serum

Method for detecting steroid hormone in serum

The invention relates to a method for detecting steroid hormones in serum, which comprises the following steps: (1) adding 0.1-20mg of magnetic solid-phase extractant into each milliliter of sample to be detected, uniformly mixing, magnetically separating, and removing liquid; adding leacheate, unif...

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Hauptverfasser: YU TAO, ZHU HUAI'EN
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
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creator YU TAO
ZHU HUAI'EN
description The invention relates to a method for detecting steroid hormones in serum, which comprises the following steps: (1) adding 0.1-20mg of magnetic solid-phase extractant into each milliliter of sample to be detected, uniformly mixing, magnetically separating, and removing liquid; adding leacheate, uniformly mixing, carrying out magnetic separation, and removing liquid; adding an eluent for elution, uniformly mixing, carrying out magnetic separation, and collecting the eluent to obtain a to-be-detected solution; (2) detecting the hormone content in the liquid to be detected by adopting liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one or a combination of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate and 17 alpha-hydroxyprogesterone; the magnetic solid-phase extraction agent comprises magnetic core ferroferric oxide, middle-layer structure silicon dioxide wrapping a magnetic core and an outer-layer mesoporous structure, and the out
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
title Method for detecting steroid hormone in serum
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