Method for detecting steroid hormone in serum

The invention relates to a method for detecting steroid hormones in serum, which comprises the following steps: (1) adding 0.1-20mg of magnetic solid-phase extractant into each milliliter of sample to be detected, uniformly mixing, magnetically separating, and removing liquid; adding leacheate, uniformly mixing, carrying out magnetic separation, and removing liquid; adding an eluent for elution, uniformly mixing, carrying out magnetic separation, and collecting the eluent to obtain a to-be-detected solution; (2) detecting the hormone content in the liquid to be detected by adopting liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one or a combination of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate and 17 alpha-hydroxyprogesterone; the magnetic solid-phase extraction agent comprises magnetic core ferroferric oxide, middle-layer structure silicon dioxide wrapping a magnetic core and an outer-layer mesoporous structure, and the out