Visual instant detection micro-fluidic chip and detection method thereof

The invention provides a visual instant detection micro-fluidic chip and a detection method thereof, and belongs to the field of micro-fluidic chip manufacturing technology and gene diagnosis. The micro-fluidic chip for visual instant detection of Ustilaginoidea virens nucleic acid integrates the functions of spore collection, rapid release and amplification of spore nucleic acid, visual detection and the like; the micro-fluidic chip is simple in structure, the detection method has the characteristics of rapidness and no need of large instruments, and rapid detection of various fungal spores, especially false smut spores, can be realized. 本发明提供了一种可视化即时检测微流控芯片及其检测方法，属于微流控芯片制造技术和基因诊断领域；本发明所述可视化即时检测稻曲病菌核酸的微流控芯片集孢子收集、孢子核酸快速释放、扩增及可视化检测等功能为一体；所述微流控芯片结构简单，其检测方法具有快速、无需大型仪器的特点，能够实现对各类真菌孢子尤其是稻曲病孢子的快速检测。