Light chain and heavy chain variable regions of anti-carbaryl monoclonal antibody and application of light chain and heavy chain variable regions
The invention discloses light chain and heavy chain variable regions of an anti-carbaryl monoclonal antibody and application of the light chain and heavy chain variable regions. The light chain variable regions of the monoclonal antibody are respectively CDR-L1, CDR-L2 and CDR-L3, and the amino acid...
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Zusammenfassung: | The invention discloses light chain and heavy chain variable regions of an anti-carbaryl monoclonal antibody and application of the light chain and heavy chain variable regions. The light chain variable regions of the monoclonal antibody are respectively CDR-L1, CDR-L2 and CDR-L3, and the amino acid sequences of the light chain variable regions are respectively shown as SEQ ID NO.5, SEQ ID NO.6 and SEQ ID NO.7; heavy chain variable regions of the monoclonal antibody are respectively CDR-H1, CDR-H2 and CDR-H3, and the amino acid sequences of the heavy chain variable regions of the monoclonal antibody are respectively shown as SEQ ID NO.8, SEQ ID NO.9 and SEQ ID NO.10. The monoclonal antibody with relatively high affinity and detection sensitivity to carbaryl has the advantages of strong specificity, high affinity and detection sensitivity and convenience and rapidness in detection, and can be used as a raw material for enzyme-linked immunosorbent assay and colloidal gold immunoassay; and a foundation can be la |
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