Bovine viral diarrhea virus, infectious rhinotracheitis virus, parainfluenza virus type 3 and mycoplasma quadruple inactivated vaccine

The invention discloses a bovine viral diarrhea virus, infectious rhinotracheitis virus, parainfluenza virus and mycoplasma quadruple inactivated vaccine. The active ingredients of the quadruple inactivated vaccine are an inactivated bovine viral diarrhea virus, an inactivated bovine infectious rhinotracheitis virus, an inactivated bovine parainfluenza virus type 3 and an inactivated mycoplasma bovis. According to the quadruple inactivated vaccine, a BVDV HH03 strain virus, an IBRV TY01 strain virus and a BPIV3LH01 strain virus are bred by adopting an MDBK cell suspension culture method; the method comprises the following steps: culturing a mycoplasma bovis CHF01 strain by adopting a Hayflash liquid culture medium; according to the method, viruses can be well cultured on the suspension culture cells, the problems that virus inoculation is uneven and even virus liquid with high titer is difficult to obtain in the adherent cell virus breeding process are solved, TCID50 is larger than 8.0, the production cost is