Method for culturing somatic embryogenesis regeneration plant of davidia involucrata
The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting t...
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creator | DAI ZHAO DUAN RONGYI DONG JIAMING LIU RUI WANG ANQI KANG YONGXIANG LINGHU GAOMAN |
description | The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting the explants by using a disinfectant, and using the disinfected explants as materials for embryogenic callus induction; (2) embryogenic callus induction culture: putting the explant treated in the step (1) into an embryogenic callus induction culture medium for culture to obtain embryogenic callus; (3) somatic embryo development induction culture: putting the embryogenic callus treated in the step (2) into a somatic embryo development induction culture medium for culture to obtain a mature somatic embryo; and (4) somatic embryo germination and transformed plant culture: putting the mature somatic embryo obtained in the step (3) into a germination culture medium for culture, and then putting the mature somatic embryo |
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(2) embryogenic callus induction culture: putting the explant treated in the step (1) into an embryogenic callus induction culture medium for culture to obtain embryogenic callus; (3) somatic embryo development induction culture: putting the embryogenic callus treated in the step (2) into a somatic embryo development induction culture medium for culture to obtain a mature somatic embryo; and (4) somatic embryo germination and transformed plant culture: putting the mature somatic embryo obtained in the step (3) into a germination culture medium for culture, and then putting the mature somatic embryo</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230331&DB=EPODOC&CC=CN&NR=115868412A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230331&DB=EPODOC&CC=CN&NR=115868412A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DAI ZHAO</creatorcontrib><creatorcontrib>DUAN RONGYI</creatorcontrib><creatorcontrib>DONG JIAMING</creatorcontrib><creatorcontrib>LIU RUI</creatorcontrib><creatorcontrib>WANG ANQI</creatorcontrib><creatorcontrib>KANG YONGXIANG</creatorcontrib><creatorcontrib>LINGHU GAOMAN</creatorcontrib><title>Method for culturing somatic embryogenesis regeneration plant of davidia involucrata</title><description>The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting the explants by using a disinfectant, and using the disinfected explants as materials for embryogenic callus induction; (2) embryogenic callus induction culture: putting the explant treated in the step (1) into an embryogenic callus induction culture medium for culture to obtain embryogenic callus; (3) somatic embryo development induction culture: putting the embryogenic callus treated in the step (2) into a somatic embryo development induction culture medium for culture to obtain a mature somatic embryo; and (4) somatic embryo germination and transformed plant culture: putting the mature somatic embryo obtained in the step (3) into a germination culture medium for culture, and then putting the mature somatic embryo</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAjxTS3JyE9RSMsvUkguzSkpLcrMS1cozs9NLMlMVkjNTSqqzE9PzUstzixWKEoFsYqAMvl5CgU5iXklCvlpCimJZZkpmYkKmXll-TmlyUDpRB4G1rTEnOJUXijNzaDo5hri7KGbWpAfn1pckJgMNKck3tnP0NDUwszCxNDI0ZgYNQARXTnF</recordid><startdate>20230331</startdate><enddate>20230331</enddate><creator>DAI ZHAO</creator><creator>DUAN RONGYI</creator><creator>DONG JIAMING</creator><creator>LIU RUI</creator><creator>WANG ANQI</creator><creator>KANG YONGXIANG</creator><creator>LINGHU GAOMAN</creator><scope>EVB</scope></search><sort><creationdate>20230331</creationdate><title>Method for culturing somatic embryogenesis regeneration plant of davidia involucrata</title><author>DAI ZHAO ; DUAN RONGYI ; DONG JIAMING ; LIU RUI ; WANG ANQI ; KANG YONGXIANG ; LINGHU GAOMAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115868412A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>DAI ZHAO</creatorcontrib><creatorcontrib>DUAN RONGYI</creatorcontrib><creatorcontrib>DONG JIAMING</creatorcontrib><creatorcontrib>LIU RUI</creatorcontrib><creatorcontrib>WANG ANQI</creatorcontrib><creatorcontrib>KANG YONGXIANG</creatorcontrib><creatorcontrib>LINGHU GAOMAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DAI ZHAO</au><au>DUAN RONGYI</au><au>DONG JIAMING</au><au>LIU RUI</au><au>WANG ANQI</au><au>KANG YONGXIANG</au><au>LINGHU GAOMAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for culturing somatic embryogenesis regeneration plant of davidia involucrata</title><date>2023-03-31</date><risdate>2023</risdate><abstract>The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting the explants by using a disinfectant, and using the disinfected explants as materials for embryogenic callus induction; (2) embryogenic callus induction culture: putting the explant treated in the step (1) into an embryogenic callus induction culture medium for culture to obtain embryogenic callus; (3) somatic embryo development induction culture: putting the embryogenic callus treated in the step (2) into a somatic embryo development induction culture medium for culture to obtain a mature somatic embryo; and (4) somatic embryo germination and transformed plant culture: putting the mature somatic embryo obtained in the step (3) into a germination culture medium for culture, and then putting the mature somatic embryo</abstract><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
title | Method for culturing somatic embryogenesis regeneration plant of davidia involucrata |
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