Method for culturing somatic embryogenesis regeneration plant of davidia involucrata

The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting t...

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Bibliographische Detailangaben
Hauptverfasser: DAI ZHAO, DUAN RONGYI, DONG JIAMING, LIU RUI, WANG ANQI, KANG YONGXIANG, LINGHU GAOMAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for culturing somatic embryogenesis regeneration plants of davidia involucrata, which comprises the following steps: (1) explant selection and disinfection: selecting immature zygotic embryos of davidia involucrata in the most timely period as explants, disinfecting the explants by using a disinfectant, and using the disinfected explants as materials for embryogenic callus induction; (2) embryogenic callus induction culture: putting the explant treated in the step (1) into an embryogenic callus induction culture medium for culture to obtain embryogenic callus; (3) somatic embryo development induction culture: putting the embryogenic callus treated in the step (2) into a somatic embryo development induction culture medium for culture to obtain a mature somatic embryo; and (4) somatic embryo germination and transformed plant culture: putting the mature somatic embryo obtained in the step (3) into a germination culture medium for culture, and then putting the mature somatic embryo