Real-time fluorescent nucleic acid isothermal amplification detection kit for prostatic cancer and special primer and probe thereof

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for prostatic cancer as well as a special primer and a probe thereof, and belongs to the technical field of tumor diagnosis. The kit provided by the invention comprises reagents for respectively and specifically detecting the following genes based on a real-time fluorescent nucleic acid isothermal amplification method: PCA3, ERG, DLX1, HOXC6, HOXC4, TDRD1, AMACR and MALAT1, and optional SPDEF and/or KLK3, the kit can be used for high-sensitivity detection of prostate cancer (including blood PSA grey region) by taking random urine of a patient as a sample to be detected, has an extremely high negative predictive value, and can be used for detection of prostate cancer (including blood PSA grey region). Therefore, unnecessary prostate needle biopsy of a negative object can be effectively avoided. 本发明公开一种前列腺癌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针，属于肿瘤诊断技术领域。提供的试剂盒包括基于实时荧光核酸恒温扩增方法的分别特异性检测以下基因的试剂：PCA3、ERG、DLX1、HOXC6、HOXC4、TDRD1、A