Eukaryotic expression vector for efficiently expressing porcine PFKFB3 gene and construction method of eukaryotic expression vector

The invention discloses an eukaryotic expression vector for efficiently expressing a porcine PFKFB3 gene and a construction method of the eukaryotic expression vector, and belongs to the field of molecular biology. Aiming at cloning of a PFKFB3 gene of a PFKFB family and construction of an eukaryotic expression vector, a specific primer containing a specific enzyme cutting site and a protective basic group is designed, PrimerSTAR high-fidelity enzyme cloning is applied to obtain a specific coding sequence, a PFKFB3 gene recombinant plasmid is constructed, specific enzyme cutting treatment is performed, and the PFKFB3 gene recombinant plasmid is connected with a linearized vector to obtain the eukaryotic expression vector pcDNA3.1 (+)-PFKFB3 for efficiently expressing the porcine PFKFB3 gene. The PFKFB3 is used as an intracellular regulatory molecule to control glycolysis in all eukaryotic cell tissues, the expression vector is used for elaborating the relationship between the glycometabolism process and infla