ShRNA of bombyx mori Bmp53 gene and application thereof

The invention discloses shRNA (short hairpin ribonucleic acid) and an expression cassette, a recombinant vector, a recombinant cell or a recombinant bacterium thereof, and further discloses application of the shRNA to bombyx mori Bmp53 gene interference. According to the invention, the wide infectio...

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Bibliographische Detailangaben
Hauptverfasser: FAN BINGYAN, SHEN XINGJIA, LUO YUHAN, ASEN, AYINUR, WANG MEIXIAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses shRNA (short hairpin ribonucleic acid) and an expression cassette, a recombinant vector, a recombinant cell or a recombinant bacterium thereof, and further discloses application of the shRNA to bombyx mori Bmp53 gene interference. According to the invention, the wide infection ability of the lentiviral vector is utilized to construct the shRNA stable expression vector of the silkworm functional gene and apply the shRNA stable expression vector to the individual level, and a new tool for silkworm gene function research is developed. 本发明公开了一种shRNA及其表达盒、重组载体、重组细胞或重组菌,还公开了该shRNA对于家蚕Bmp53基因干涉的应用。本发明利用慢病毒载体的广泛感染能力,构建家蚕功能基因的shRNA稳定表达载体并应用到个体水平,开发了一种家蚕基因功能研究的新工具。