Beta-caryophyllene synthase mutant, coding gene and application of beta-caryophyllene synthase mutant in beta-caryophyllene synthesis

The invention discloses a beta-caryophyllene synthase mutant, a coding gene and application of the beta-caryophyllene synthase mutant in synthesis of beta-caryophyllene. The invention relates to the technical field of biology, in particular to a beta-caryophyllene synthase mutant, a coding gene and application of the beta-caryophyllene synthase mutant in beta-caryophyllene synthesis. The amino acid sequence of the beta-caryophyllene synthase mutant obtained through error-prone PCR is a sequence 2 in a sequence table. The saccharomyces cerevisiae strain SQ3-6-ECPS11 is used as a base bacterium, the catalytic efficiency of the beta-caryophyllene synthase is improved through directional transformation, 600-800 mg/L beta-caryophyllene can be generated in 96 hours of fermentation when the beta-caryophyllene synthase is synthesized by using the saccharomyces cerevisiae strain SQ3-6-ECPS11 as the base bacterium, and the yield index has important industrial application potential. 本发明公开了β-石竹烯合酶突变体和编码基因及其在β-石竹烯合成中的应用。本