Nitrite reductase as well as encoding gene, expression and application thereof

The invention firstly relates to the field of gene engineering, secondly relates to the biotechnical field of enzyme genes and enzyme application, and provides a novel nitrite reductase gene, and the optimum pH value of nitrite reductase coded by the novel nitrite reductase gene is 5.5; the optimum...

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Bibliographische Detailangaben
Hauptverfasser:	PAN YAYING, SONG LI, JI GENGSHENG, WEI WANQI, ZHONG HAOYU, LI QIANG, CHEN YANPING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention firstly relates to the field of gene engineering, secondly relates to the biotechnical field of enzyme genes and enzyme application, and provides a novel nitrite reductase gene, and the optimum pH value of nitrite reductase coded by the novel nitrite reductase gene is 5.5; the optimum reaction temperature is 38 DEG C; after being treated in a 0.05 M buffer solution with the pH value of 5.5 for 12 hours, 90% or above of activity can still be kept; the nitrite detection enzyme reagent can still keep 75.3% of relative enzyme activity after being used for 20 times; the method has the characteristics of good nitrite hydrolysis, high sensitivity and high specificity of enzyme reaction, can be used as a nitrite detection reagent, and can also be used as a feed or food additive to be applied to feed and food industries. 本发明首先涉及基因工程领域，其次涉及酶基因和酶应用的生物技术领域，本发明提供了一个新的亚硝酸盐还原酶基因，其编码的亚硝酸盐还原酶最适pH5.5；最适反应温度38℃；经0.05MpH5.5的缓冲液中处理12h，仍能保持90％以上的活性；制成亚硝酸盐检测酶试剂，使用20次仍能保持75.3％的相对酶活；具有较好的水解亚硝酸盐，具有酶反应的高灵敏度和高特异性的特点，可作为亚硝酸