Chimeric antigen receptor targeting MAGE-A1 and capable of automatically secreting CD47 scFv and application of chimeric antigen receptor

The invention relates to the technical field of biological medicines, and provides a chimeric antigen receptor modified T lymphocyte with a self-secretion function as well as a preparation method and application of the chimeric antigen receptor modified T lymphocyte. The invention discloses a chimeric antigen receptor targeting MAGE-A1 and capable of automatically secreting CD47scFv. T cells modified by the chimeric antigen receptor can specifically kill tumor cells with high expression of MAGE-A1 under the condition of self-activation, a CD47scFv antibody is automatically secreted, the immunosuppression effect of a tumor microenvironment is weakened, and the killing effect of autoimmune cells on tumors is promoted. 本申请涉及生物医药技术领域，提供一种具有自分泌功能的嵌合抗原受体修饰的T淋巴细胞及其制备方法和应用。本申请公开了一种靶向MAGE-A1且自分泌CD47scFv的嵌合抗原受体，本申请的嵌合抗原受体修饰的T细胞在自身活化的情况下，还可特异杀伤MAGE-A1高表达的肿瘤细胞，并自分泌CD47scFv抗体，减弱肿瘤微环境的免疫抑制作用，促进自体免疫细胞对肿瘤的杀伤作用。