Clostridium perfringens alpha toxin nano antibody and application thereof

The invention provides a clostridium perfringens alpha toxin nano-antibody and application thereof, and belongs to the technical field of bioengineering, and the amino acid sequence of the clostridium perfringens alpha toxin nano-antibody is as shown in SEQ ID No.1. The clostridium perfringens alpha toxin nano antibody provided by the invention can be specifically combined with clostridium perfringens alpha toxin protein, and the lowest sensitivity to the protein is 0.625 mu g/ml; the clostridium perfringens alpha toxin in chicken and alpaca faeces can be detected by an ELISA method, and the sensitivity is 2 mu g/ml. 本发明提供了一种产气荚膜梭菌α毒素纳米抗体及其应用，属于生物工程技术领域，所述产气荚膜梭菌α毒素纳米抗体的氨基酸序列如SEQ ID No.1所示。本发明提供的产气荚膜梭菌α毒素纳米抗体能够特异性结合产气荚膜梭菌α毒素蛋白，对该蛋白的灵敏度为最低为0.625μg/ml；能以ELISA的方法检测鸡和羊驼粪便中的产气荚膜梭菌α毒素，灵敏度为2μg/ml。