Regeneration method taking honeysuckle flower anther as explant

The invention relates to the technical field of plant tissue culture, and provides a regeneration method taking honeysuckle flower anther as an explant, which comprises the following steps: S1, preparing a callus induction culture medium M1, a callus induction culture medium M2, a differential culture medium and a rooting culture medium; s2, honeysuckle explants are obtained and sterilized, and honeysuckle anther is obtained; s3, culturing the honeysuckle flower anther in the callus induction culture medium M1, and carrying out subculture in the callus induction culture medium M2 until embryogenic callus is generated; s4, carrying out differentiation culture on the embryogenic callus to form a regenerated plant, and transferring the regenerated plant to a rooting culture medium for culture to obtain a honeysuckle tissue culture seedling. According to the culture method provided by the invention, the honeysuckle flower anther can be directly induced to generate the embryogenic callus, the production steps are