Method for detecting content of (1-3)-beta-D-glucan and application thereof

The invention relates to the technical field of in-vitro diagnosis, in particular to a method for detecting the content of (1-3)-beta-D-glucan and application of the method. The detection method provided by the invention comprises the following steps: carrying out enzymolysis treatment, acidolysis treatment and neutralization treatment on a sample, and then detecting the content of (1-3)-beta-D-glucan in the sample by utilizing an ELISA (Enzyme-Linked Immunosorbent Assay) immunodetection method established by an anti-BG monoclonal antibody. The method is simple to operate, has the sensitivity equivalent to that of a tachypleus amebocyte lysate, is matched with a full-automatic chemiluminiscence instrument for use, can realize random automatic detection of samples, and has the advantages of simplicity, rapidness and accuracy in operation. 本发明涉及体外诊断技术领域，尤其涉及检测(1-3)-β-D-葡聚糖含量的方法及其应用。本发明提供的检测方法包括对样本进行酶解处理、酸解处理和中和处理后，再利用抗BG的单克隆抗体建立的ELISA免疫检测方法检测样本中(1-3)-β-D-葡聚糖的含量。该方法操作简单且灵敏度与鲎试剂相当，与全自动化学发光仪仪器配合使用，可实现对样本的随机自动化检测，具