Human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a
The invention discloses a human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a. The human rhinovirus type A and type C detection system comprises a reverse transcription recombinant polymerase amplification system and a CRISPR/Cas12a cutting system, and the RT-RAA is...
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| creator | JIAO SULI LI YONGDONG XU RONG NI HONGXIA ZHANG DANDAN |
| description | The invention discloses a human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a. The human rhinovirus type A and type C detection system comprises a reverse transcription recombinant polymerase amplification system and a CRISPR/Cas12a cutting system, and the RT-RAA isothermal amplification system comprises RT-RAA primer pairs of human rhinoviruses type A and type C, RNase inhibitors, template RNA, nuclease-free water, a buffer solution and magnesium acetate; the CRISPR/Cas12a cutting system comprises specific crRNA (Complementary Ribonucleic Acid) aiming at human rhinoviruses A and C, Cas12a protein, ssDNA (Deoxyribose Nucleic Acid) reporter molecules, an NEB2.1 buffer system, an RNase inhibitor and nuclease-free water. The specific crRNA is designed and synthesized aiming at conserved sequence fragments of human rhinoviruses A and C; the ssDNA reporter comprises an ssDNAFAM reporter used for fluorescence detection of a microplate reader, a real-time fluorescence detector or an |
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The human rhinovirus type A and type C detection system comprises a reverse transcription recombinant polymerase amplification system and a CRISPR/Cas12a cutting system, and the RT-RAA isothermal amplification system comprises RT-RAA primer pairs of human rhinoviruses type A and type C, RNase inhibitors, template RNA, nuclease-free water, a buffer solution and magnesium acetate; the CRISPR/Cas12a cutting system comprises specific crRNA (Complementary Ribonucleic Acid) aiming at human rhinoviruses A and C, Cas12a protein, ssDNA (Deoxyribose Nucleic Acid) reporter molecules, an NEB2.1 buffer system, an RNase inhibitor and nuclease-free water. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a |
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