Human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a
The invention discloses a human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a. The human rhinovirus type A and type C detection system comprises a reverse transcription recombinant polymerase amplification system and a CRISPR/Cas12a cutting system, and the RT-RAA is...
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Zusammenfassung: | The invention discloses a human rhinovirus type A and type C detection system based on RT-RAA and CRISPR/Cas12a. The human rhinovirus type A and type C detection system comprises a reverse transcription recombinant polymerase amplification system and a CRISPR/Cas12a cutting system, and the RT-RAA isothermal amplification system comprises RT-RAA primer pairs of human rhinoviruses type A and type C, RNase inhibitors, template RNA, nuclease-free water, a buffer solution and magnesium acetate; the CRISPR/Cas12a cutting system comprises specific crRNA (Complementary Ribonucleic Acid) aiming at human rhinoviruses A and C, Cas12a protein, ssDNA (Deoxyribose Nucleic Acid) reporter molecules, an NEB2.1 buffer system, an RNase inhibitor and nuclease-free water. The specific crRNA is designed and synthesized aiming at conserved sequence fragments of human rhinoviruses A and C; the ssDNA reporter comprises an ssDNAFAM reporter used for fluorescence detection of a microplate reader, a real-time fluorescence detector or an |
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