Protease with carnosine hydrolase function and application thereof in synthesis of L-carnosine

The invention provides protease with a carnosine hydrolase function and application of the protease in synthesis of L-carnosine. Wherein the protease with the carnosine hydrolase function comprises a sequence as shown in SEQ ID NO: 6. The protease provided by the invention has the advantages of high enzymatic activity and high enzymatic reaction level. According to the method, the L-carnosine is catalytically synthesized by adopting a biological enzyme method, the content of the L-carnosine in the conversion liquid is 100-125g/L, the conversion rate is greater than 65% in terms of L-histidine, and the method has the advantages of high conversion rate, good repeatability and short conversion time. 本申请提供一种具有肌肽水解酶功能的蛋白酶及其在L-肌肽合成中的应用。其中，所述具有肌肽水解酶功能的蛋白酶包括SEQ ID NO：6所示的序列。本申请的蛋白酶具有酶活性高、酶促反应水平高的优点。本申请采用生物酶法催化合成L-肌肽，转化液中的L-肌肽含量在100-125g/L之间，以L-组氨酸计，转化率＞65％，具有转化率高、重复性好、转化时间短的优点。