Method for purifying recombinant urate oxidase

The invention relates to a purification method of recombinant urate oxidase, and solves the technical problems of low product purity, low activity and to-be-improved quality in the existing purification method of oxidase. According to the purification method of the recombinant urate oxidase, a complementary chromatography technology is alternately applied, a three-step purification process is reasonably linked, and the recombinant urate oxidase stock solution is obtained by using affinity chromatography, ion exchange and molecular sieve chromatography methods for purification, so that the production cost is reduced, the protein yield is improved, and the product quality can be further improved. According to the purification method, the RP-HPLC purity of the purified recombinant urate oxidase protein stock solution reaches 98%, the enzyme specific activity reaches 23 EAU/mg, and the purification process is simple and suitable for large-scale amplification. 本发明涉及一种重组尿酸氧化酶的纯化方法，解决现有氧化酶的纯化方法存在产品纯度低、活性低，质量有待提高的技术问