ELISA (enzyme-linked immunosorbent assay) kit for detecting triclosan residue based on nano antibody and application of ELISA kit

The invention provides an ELISA (enzyme-linked immuno sorbent assay) kit for detecting triclosan residue based on a nano antibody and application of the ELISA kit. The kit comprises a kit body, a detachable elisa plate arranged in the kit body and a reagent arranged in the kit body, wherein each hole of the elisa plate is coated with a triclosan artificial antigen, and the reagent comprises a nano antibody as shown in SEQ ID NO: 1, a triclosan standard solution, an elisa secondary antibody, a PBS (Phosphate Buffer Solution), a PBST (Phosphate Buffer Solution) washing solution, a developing solution, a reaction stop solution and the like. The triclosan residue in water, soil, vegetables and other environments can be accurately and sensitively detected by using the mobile phone number, the pretreatment process of the sample is simple, the time consumption is low, a large number of samples can be detected at the same time, and the sample detection cost is far lower than that of a traditional instrument detection