Nucleic acid detection method based on CRISPR (clustered regularly interspaced short palindromic repeats) system trans-cleavage and graphene field effect transistor

The invention discloses a nucleic acid detection method based on CRISPR (clustered regularly interspaced short palindromic repeats)-Cas trans-cleavage and a graphene field effect transistor. The method comprises the following steps: amplifying a Cas12b trans-cleavage detection signal in a solution by using a graphene field effect transistor, and detecting monkey pox virus nucleic acid. Cas12b-sgRNA has relatively high activity and specificity on DNA fragments, detection signal amplification is carried out based on a graphene field effect transistor, ultra-sensitive detection of target DNA is realized, the detection time is short, the sensitivity is high, and the Cas12b-sgRNA can be better applied to rapid screening of monkey pox viruses. 本发明公开了一种基于CRISPR-Cas反式切割和石墨烯场效应晶体管的核酸检测方法。所述方法包括：使用石墨烯场效应晶体管放大溶液中Cas12b反式切割检测信号，检测猴痘病毒核酸。利用Cas12b-sgRNA对DNA片段具有较高的活性和特异性，基于石墨烯场效应晶体管进行检测信号放大，实现靶DNA的超灵敏检测，检测时间短、灵敏度高，可以更好地应用于猴痘病毒的快速筛检。